When making use of BaseSpace Sequence Hub PrepTab, i5 sequences needs to be entered into these software program while in the ahead orientation in all instances and for all instrument sorts.
Refer to Index two (i5) Orientation For more info regarding how to enter i5 bases over the sample sheet in ahead or reverse complement orientation.
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When using LRM, i5 sequences has to be entered into these program inside the forward orientation in all conditions and for all instrument forms.
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The sequences shown are what is entered into the ultimate sample sheet. Kit definitions have the i5 from the forward orientation. The i5 sequences will be properly reverse complemented and displayed from the IEM interface depending on instrument/reagent type.
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Index 2: the i5 index column is during the reverse complement orientation (tend to be the reverse complement on the i5 indexes in the sample sheet).